Improving enzymatic saccharification of hardwood through lignin modification by carbocation scavengers and the underlying mechanisms.
Identifieur interne : 000891 ( Main/Exploration ); précédent : 000890; suivant : 000892Improving enzymatic saccharification of hardwood through lignin modification by carbocation scavengers and the underlying mechanisms.
Auteurs : Qiulu Chu [République populaire de Chine] ; Kai Song [République populaire de Chine] ; Jing Wang [République populaire de Chine] ; Jinguang Hu [Canada] ; Xueyan Chen [République populaire de Chine]Source :
- Bioresource technology [ 1873-2976 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical : Cellulose, Lignin, Steam.
- Biomass, Hydrolysis.
Abstract
In this work, the beneficial effect of carbocation scavenger additives on hardwood pretreatment was revealed by significantly improved biomass saccharification: cellulose hydrolysis yield was increased by over 15% after steam pretreatment of poplar, while that was enhanced by more than 48% after dilute acid pretreatment. Besides, the relative contributions of lignin towards enzyme binding and physical barrier effect for proposed mechanisms were quantified. Results indicated that the addition of carbocation scavenger, 2-naphthol-7-sulfonate, resulted in acid groups incorporation of 62.36 mmol/kg to lignin, which mitigated enzyme non-productive binding. Moreover, enlarged biomass porosity and reduced surface lignin coverage were detected through BET and XPS analysis, respectively, which mostly related to the diminished physical barrier effect of lignin. As a result, the lignin inhibitions were significantly suppressed through the addition of carbocation scavenger, giving rise to significantly improved enzymatic hydrolysis of hardwood.
DOI: 10.1016/j.biortech.2019.122216
PubMed: 31605916
Affiliations:
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Electronic address: ksong@njfu.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Environmental Engineering, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037</wicri:regionArea><wicri:noRegion>Nanjing 210037</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Jing" sort="Wang, Jing" uniqKey="Wang J" first="Jing" last="Wang">Jing Wang</name><affiliation wicri:level="1"><nlm:affiliation>Guangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangdong Academy of Forestry, Guangzhou 510520, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangdong Academy of Forestry, Guangzhou 510520</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Hu, Jinguang" sort="Hu, Jinguang" uniqKey="Hu J" first="Jinguang" last="Hu">Jinguang Hu</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemical and Petroleum Engineering, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1Z4, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Chemical and Petroleum Engineering, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1Z4</wicri:regionArea><orgName type="university">Université de Calgary</orgName><placeName><settlement type="city">Calgary</settlement><region type="state">Alberta</region></placeName></affiliation></author><author><name sortKey="Chen, Xueyan" sort="Chen, Xueyan" uniqKey="Chen X" first="Xueyan" last="Chen">Xueyan Chen</name><affiliation wicri:level="1"><nlm:affiliation>School of Agricultural Equipment Engineering, Jiangsu University, Zhenjiang 212013, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Agricultural Equipment Engineering, Jiangsu University, Zhenjiang 212013</wicri:regionArea><wicri:noRegion>Zhenjiang 212013</wicri:noRegion></affiliation></author></analytic><series><title level="j">Bioresource technology</title><idno type="eISSN">1873-2976</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biomass (MeSH)</term><term>Cellulose (MeSH)</term><term>Hydrolysis (MeSH)</term><term>Lignin (MeSH)</term><term>Steam (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Biomasse (MeSH)</term><term>Cellulose (MeSH)</term><term>Hydrolyse (MeSH)</term><term>Lignine (MeSH)</term><term>Vapeur (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Cellulose</term><term>Lignin</term><term>Steam</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biomass</term><term>Hydrolysis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biomasse</term><term>Cellulose</term><term>Hydrolyse</term><term>Lignine</term><term>Vapeur</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this work, the beneficial effect of carbocation scavenger additives on hardwood pretreatment was revealed by significantly improved biomass saccharification: cellulose hydrolysis yield was increased by over 15% after steam pretreatment of poplar, while that was enhanced by more than 48% after dilute acid pretreatment. Besides, the relative contributions of lignin towards enzyme binding and physical barrier effect for proposed mechanisms were quantified. Results indicated that the addition of carbocation scavenger, 2-naphthol-7-sulfonate, resulted in acid groups incorporation of 62.36 mmol/kg to lignin, which mitigated enzyme non-productive binding. Moreover, enlarged biomass porosity and reduced surface lignin coverage were detected through BET and XPS analysis, respectively, which mostly related to the diminished physical barrier effect of lignin. As a result, the lignin inhibitions were significantly suppressed through the addition of carbocation scavenger, giving rise to significantly improved enzymatic hydrolysis of hardwood.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Automated" Owner="NLM"><PMID Version="1">31605916</PMID><DateCompleted><Year>2019</Year><Month>11</Month><Day>04</Day></DateCompleted><DateRevised><Year>2019</Year><Month>11</Month><Day>04</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2976</ISSN><JournalIssue CitedMedium="Internet"><Volume>294</Volume><PubDate><Year>2019</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Bioresource technology</Title><ISOAbbreviation>Bioresour Technol</ISOAbbreviation></Journal><ArticleTitle>Improving enzymatic saccharification of hardwood through lignin modification by carbocation scavengers and the underlying mechanisms.</ArticleTitle><Pagination><MedlinePgn>122216</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S0960-8524(19)31446-4</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.biortech.2019.122216</ELocationID><Abstract><AbstractText>In this work, the beneficial effect of carbocation scavenger additives on hardwood pretreatment was revealed by significantly improved biomass saccharification: cellulose hydrolysis yield was increased by over 15% after steam pretreatment of poplar, while that was enhanced by more than 48% after dilute acid pretreatment. Besides, the relative contributions of lignin towards enzyme binding and physical barrier effect for proposed mechanisms were quantified. Results indicated that the addition of carbocation scavenger, 2-naphthol-7-sulfonate, resulted in acid groups incorporation of 62.36 mmol/kg to lignin, which mitigated enzyme non-productive binding. Moreover, enlarged biomass porosity and reduced surface lignin coverage were detected through BET and XPS analysis, respectively, which mostly related to the diminished physical barrier effect of lignin. As a result, the lignin inhibitions were significantly suppressed through the addition of carbocation scavenger, giving rise to significantly improved enzymatic hydrolysis of hardwood.</AbstractText><CopyrightInformation>Copyright © 2019 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chu</LastName><ForeName>Qiulu</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>School of Agricultural Equipment Engineering, Jiangsu University, Zhenjiang 212013, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Song</LastName><ForeName>Kai</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Environmental Engineering, College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China. Electronic address: ksong@njfu.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Jing</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Guangdong Provincial Key Laboratory of Silviculture, Protection and Utilization, Guangdong Academy of Forestry, Guangzhou 510520, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hu</LastName><ForeName>Jinguang</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Chemical and Petroleum Engineering, University of Calgary, 2500 University Dr. NW, Calgary, AB T2N 1Z4, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Xueyan</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>School of Agricultural Equipment Engineering, Jiangsu University, Zhenjiang 212013, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>09</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Bioresour Technol</MedlineTA><NlmUniqueID>9889523</NlmUniqueID><ISSNLinking>0960-8524</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013227">Steam</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-34-6</RegistryNumber><NameOfSubstance UI="D002482">Cellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="N">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002482" MajorTopicYN="Y">Cellulose</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="Y">Lignin</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013227" MajorTopicYN="N">Steam</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Carbocation scavenger</Keyword><Keyword MajorTopicYN="N">Enzymatic hydrolysis</Keyword><Keyword MajorTopicYN="N">Lignin modification</Keyword><Keyword MajorTopicYN="N">Non-productive binding</Keyword><Keyword MajorTopicYN="N">Physical barrier</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>08</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>09</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>09</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>10</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>11</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>10</Month><Day>13</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31605916</ArticleId><ArticleId IdType="pii">S0960-8524(19)31446-4</ArticleId><ArticleId IdType="doi">10.1016/j.biortech.2019.122216</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li><li>République populaire de Chine</li></country><region><li>Alberta</li><li>Guangdong</li></region><settlement><li>Calgary</li><li>Jiangmen</li></settlement><orgName><li>Université de Calgary</li></orgName></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Chu, Qiulu" sort="Chu, Qiulu" uniqKey="Chu Q" first="Qiulu" last="Chu">Qiulu Chu</name></noRegion><name sortKey="Chen, Xueyan" sort="Chen, Xueyan" uniqKey="Chen X" first="Xueyan" last="Chen">Xueyan Chen</name><name sortKey="Song, Kai" sort="Song, Kai" uniqKey="Song K" first="Kai" last="Song">Kai Song</name><name sortKey="Wang, Jing" sort="Wang, Jing" uniqKey="Wang J" first="Jing" last="Wang">Jing Wang</name></country><country name="Canada"><region name="Alberta"><name sortKey="Hu, Jinguang" sort="Hu, Jinguang" uniqKey="Hu J" first="Jinguang" last="Hu">Jinguang Hu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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